FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2476_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2476_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260632
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC16380.6284723288007612No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG11790.4523619509499984No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCATAGG3100.11894164952883758No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCAAAGC2610.10014119524847294No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGC150.002227953969.960487
GTCTTAG200.006956285352.4804271
TCGGGGC200.00696156752.47035610
CACGAGC200.00696156752.47035616
ACCAGGC200.00696156752.4703562
CGAGCGC200.00696156752.47035618
GGCACCT350.001252730540.00811827
AGGGCGT552.983591E-431.82464237
CTTAAGG450.00431431631.0935463
CCCTATC450.00431431631.0935462
TAATACT802.4698038E-630.6077084
CCGCCAG703.4712182E-530.01185232
CGCCAGG604.9623486E-429.17818833
AGCCGCC755.564029E-528.00568430
GTTATAT500.00721341827.9895611
AGGTAGT500.0072201827.984194
CAGGGCG657.9333136E-426.92854136
TTGTACT657.9689897E-426.9078754
CTGGGGT657.9689897E-426.9078754
TAGCCGC808.633499E-526.2553329
Produced by FastQC (version 0.11.2)