FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2475_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2475_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169126
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6650.3931979707437059No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG5780.34175703321783757No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1830.10820335134751605No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCC200.006946245752.491573
CCCAAGA450.004301552731.1061152
TAAGACA500.007198897727.9955044
GTACACT657.938576E-426.9187554
TGCGGAG700.001225487624.98859612
GTATATA750.001825162823.3364891
TCCACAC750.001828308623.3295863
ACACTGT750.001828308623.3295866
TACACTG750.001828308623.3295865
TAAGCCA901.9253684E-423.32268746
AACCGAG850.00378760620.5788427
AGTACAC900.005263519519.4413223
CTGAGCA900.00527253219.4355749
AGTGTTC900.00527253219.4355748
ATGCCGT950.007155715518.4344558
GATTGCG950.007192480418.41809542
CTCGTAT1200.0013308117.50754553
CGATTGC1000.0096497117.50236741
TGTACGA1000.0096497117.50236737
ATATTGT1200.001333454217.5023671
Produced by FastQC (version 0.11.2)