FastQCFastQC Report
Tue 16 Apr 2019
H7K37BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K37BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36454027
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG952769526.136193403269274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC25200.019.5425613
TGAACTC24800.019.4448620
CGTCTGA25350.019.0167716
ACGTCTG25800.018.95431915
CACACGT26950.018.27353912
AGGGGGG33350.017.9291521
CTGAACT27350.017.50041219
GAACTCC28500.016.79854221
GCACACG30850.016.52912511
CTCCAGT26000.016.4045724
AGCACAC32550.015.55897810
CAGTCAC23300.014.71076227
AACTCCA33800.013.75185322
CACGTCT38950.013.2722514
AGAGCAC41500.012.6238488
GGGGTTA7050.012.3996741
CCAGTCA31000.011.84585426
GGGGGTA4308.8206434E-811.3846331
GCATTCC14500.010.8430083
GGGCGTA3702.544767E-510.3956191
Produced by FastQC (version 0.11.5)