FastQCFastQC Report
Tue 16 Apr 2019
H7K37BGXB_n01_input_NLP7_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K37BGXB_n01_input_NLP7_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26585257
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1095970.4122472842748896No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT312880.11768928921770437TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT71550.037.27412851
TATGCCG75600.035.9255148
GTATGCC80150.033.9304447
CCGTCTT83700.032.2397752
CGTATGC85800.031.89987846
CCGTACG97400.028.24418433
ATGCCGT96450.028.08676549
CGTACGT98850.027.86549634
ACCGTAC102450.026.78348532
TCTCGTA92200.026.57275843
GTACGTA105800.026.4317935
ATCTCGT97150.025.11111842
TACGTAA112100.024.75950836
TGCTTGA112200.024.45613960
TCGTATG124800.022.68850745
GCTTGAA120800.022.6572861
CTCGTAT115800.022.305944
TGGGGGG75200.021.180481
TGCCGTC131700.020.86172150
ACGTAAT138600.019.84892537
Produced by FastQC (version 0.11.5)