FastQCFastQC Report
Tue 16 Apr 2019
H7K37BGXB_n01_input_NLP7_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K37BGXB_n01_input_NLP7_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48632797
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1897980.39026749787802667No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG625530.12862307713866428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG80450.030.27907448
GTATGCC83100.030.07215947
GCCGTCT79650.027.7711251
CGTATGC91000.026.73073246
GTCACGT117550.024.20613929
CGTTTCG124400.023.23951733
TGGGGGG194300.023.0978491
GTTTCGG131000.022.33586934
ATGCCGT115250.021.07574349
AGTCACG149800.019.11172928
CCGTCTT123950.018.4950752
TCTCGTA115600.018.28683343
ATCTCGT128300.017.02233542
ACGTTTC176050.016.63999732
AGGGGGG219550.016.4073431
CTCGTAT141950.015.87866144
TCGTATG151450.015.78398345
CACGTTT205850.014.84302131
TGCCGTC158650.014.71472950
CAGTCAC248750.011.66401827
Produced by FastQC (version 0.11.5)