FastQCFastQC Report
Tue 16 Apr 2019
H7K37BGXB_n01_5_NLP7_novus_R5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K37BGXB_n01_5_NLP7_novus_R5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13095288
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG581340.4439306718569305No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139760.1067254114609774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC29700.032.5251646
GTATGCC30850.031.7665347
TCTCGTA31200.028.1567643
TATGCCG34900.027.97943948
GCCGTCT32750.027.25183551
TCGTATG34850.026.81474745
CTCGTAT32550.026.77401744
CCGTCTT33700.026.3796552
TGGGGGG44600.024.7241951
ATCTCGT35000.024.59983842
ATGCCGT40650.023.84978349
ACATGTC47700.022.01226632
GCTTGAA44850.020.9928661
TGCCGTC46050.020.74912850
TGCTTGA44100.020.71462460
GTCACAT52400.020.30513429
ATGTCAG53100.019.90616434
CACATGT55050.019.39103931
CATGTCA55850.019.17631333
ACACGTC58400.018.21787613
Produced by FastQC (version 0.11.5)