FastQCFastQC Report
Tue 16 Apr 2019
H7K37BGXB_n01_5_NLP7_ab_R5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K37BGXB_n01_5_NLP7_ab_R5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24592060
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1414630.5752385119424724No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT408160.1659722690982374TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA88650.039.5603436
GTATGCC90600.038.7083347
CGTATGC91050.038.44013646
CACGTGG96500.036.8489531
TCACGTG101350.035.0163730
GTCACGT103150.034.60883729
TCTCGTA93200.034.17308443
TATGCCG109450.032.2012948
AGTCACG116450.030.62609328
TCGTATG119500.029.84497345
CTCGTAT112500.029.83533744
GCCTTAT121100.028.0638937
GTGGCCT134050.026.84075434
TATCTCG119850.026.63309741
ATCTCGT118650.026.54826242
CCGTCTT133700.026.09992252
GCCGTCT136150.025.6042951
ATGCCGT138800.025.3169249
ACGTGGC144900.024.8787932
CAGTCAC151650.023.54025527
Produced by FastQC (version 0.11.5)