FastQCFastQC Report
Tue 16 Apr 2019
H7K37BGXB_n01_4_NLP7_novus_R4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K37BGXB_n01_4_NLP7_novus_R4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13822845
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG527140.38135420023880756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG43900.025.357161
GTATGCC24100.016.84650647
CGTATGC24050.015.71728646
AGTTCCG28950.013.78221134
TATGCCG28500.013.63135948
GTTCCGT29000.013.39652935
GCCGTCT29500.012.69489551
TCTCGTA28450.012.67133243
CACAGTT31550.012.31336731
TTCCGTA33600.011.14597636
AGGGGGG60100.011.0084291
CCGTCTT34850.010.94681752
TCGTATG40150.09.93775345
CTCGTAT39800.09.84930144
ATGCCGT41350.09.73399649
ATCTCGT37450.09.43923742
TCCGTAT41900.09.27219537
TATCTCG40550.08.89030841
TCACAGT45700.08.8836930
ACGTCTG50300.08.27985515
Produced by FastQC (version 0.11.5)