FastQCFastQC Report
Tue 16 Apr 2019
H7K37BGXB_n01_4_NLP7_ab_R4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K37BGXB_n01_4_NLP7_ab_R4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22000796
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG648220.29463479412290355No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT234660.10665977721896973TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC68600.029.89825846
GTATGCC69300.029.34385947
TATGCCG71600.028.4498148
GCCGTCT75700.027.3249951
CCGTCTT79700.025.82185452
TCTCGTA68800.024.97770943
GTCACGT88300.023.22717729
CACGTGA90800.022.70324931
TCACGTG90600.022.6373230
TGGGGGG65050.021.6337151
ATCTCGT78150.021.3624942
ATGCCGT105500.019.63996149
CGTGAAA106950.019.5369633
TGCTTGA106600.019.01037860
GTGAAAC113750.018.33839434
TCGTATG114350.018.33407845
GCTTGAA112600.018.0596461
CGATCTC94650.017.93389940
CTCGTAT107800.017.37010444
AGTCACG120850.016.8841628
Produced by FastQC (version 0.11.5)