FastQCFastQC Report
Tue 16 Apr 2019
H7K37BGXB_n01_3_NLP7_novus_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K37BGXB_n01_3_NLP7_novus_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17917452
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG702780.3922321097888249No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG188590.10525492129126396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG61750.024.377211
CGTATGC21650.018.2681946
GTATGCC24950.016.8340347
TCTCGTA25950.014.43138743
AGGGGGG90750.014.3113241
ACAGTCA30550.013.63311232
TATGCCG31300.013.53041648
CACACGT38700.011.75617812
GCCGTCT36100.011.6344751
ACGTCTG40700.011.35045615
ACACGTC41550.011.11825613
GCACACG41600.010.85253911
CCGTCTT40100.010.82305452
CTCGTAT42150.09.96437744
ATCTCGT37000.09.93239742
TCGTATG45850.09.84737545
CGTCTGA47350.09.83035216
ATGCCGT46950.09.54216549
CACAGTC44150.09.43342531
TGAACTC53550.09.01902220
Produced by FastQC (version 0.11.5)