FastQCFastQC Report
Tue 16 Apr 2019
H7K37BGXB_n01_3_NLP7_ab_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K37BGXB_n01_3_NLP7_ab_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12038678
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG552020.4585387199491506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21200.020.30668846
GTATGCC21550.020.1393847
GTCACGT22400.019.8430929
TGGGGGG33550.019.7206081
ACGTCCG23600.018.53790532
TCTCGTA22900.017.88187443
TCACGTC26200.017.23203730
CACGTCC26600.016.70983331
TATGCCG25700.016.61464748
AGGGGGG32500.015.9415071
TCCGCAC27950.015.65295135
CACATCT29700.013.905375539
GCCGTCT30500.013.77037751
ATCTCGT29600.013.71621642
CATCTCG30500.013.65579441
CCGTCTT31700.013.028337552
CGTCCGC34550.012.76411133
ACGTCTG39250.011.0565915
ACACGTC39250.010.96737913
CTCGTAT44300.010.82392744
Produced by FastQC (version 0.11.5)