FastQCFastQC Report
Tue 16 Apr 2019
H7K37BGXB_n01_2_NLP7_novus_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K37BGXB_n01_2_NLP7_novus_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21077328
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1278190.606428860432404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG75700.022.7057761
CGTATGC23450.022.08956744
GTATGCC24750.021.353645
TCTCGTA30300.015.7093241
TATGCCG36500.014.67134746
CCGTCTT43300.012.60985850
GCCGTCT45750.012.16449
ACCTTGT45650.012.03696132
ATCTCGT42300.011.74885940
TGTAATC42400.011.63896636
TCGTATG49350.011.3473943
CTCGTAT47150.010.76337442
ACGTCTG52750.010.74813415
ATGCCGT53350.010.49677747
TGCTTGA49750.010.48251858
AGGGGGG124250.010.4526951
CACACGT54250.010.38646312
ACACGTC54050.010.16589213
CGTCTGA58050.09.766891516
GCTTGAA54500.09.63312559
Produced by FastQC (version 0.11.5)