FastQCFastQC Report
Tue 16 Apr 2019
H7K37BGXB_n01_2_NLP7_ab_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K37BGXB_n01_2_NLP7_ab_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15982062
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG391070.24469308153103148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT24100.024.39810829
CGTATGC26550.022.80538244
GTATGCC27100.022.08431445
TCTCGTA26850.020.98676741
TGGGGGG30800.018.4132961
TATGCCG34500.017.1443946
GAGATCT34750.015.81294737
AGATCTC36550.014.74687438
GCCGTCT41150.014.45906749
CCGTCTT43200.014.09704650
ATCTCGT39800.013.36653940
CACGTAG43600.013.24524631
ACACGTC47500.012.96744613
ACGTCTG47650.012.92662515
TCACGTA49650.012.12468330
CACACGT50000.012.03909512
AGTCACG50000.011.89992428
CGTCTGA51750.011.42916416
TGAACTC55150.011.29589820
CTCGTAT59250.010.86912942
Produced by FastQC (version 0.11.5)