FastQCFastQC Report
Tue 16 Apr 2019
H7K37BGXB_n01_1_NLP7_novus_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K37BGXB_n01_1_NLP7_novus_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23399785
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG741920.3170627422431445No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC426690.18234782926424323TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC72450.049.03329544
TCTCGTA68700.047.3794141
TATGCCG80700.044.0206946
CTCGTAT80650.041.96492842
TCGTATG85750.041.632343
GTATGCC88000.040.4087545
ATCTCGT82250.038.8925440
CCGTCTT90400.038.75561550
GCCGTCT93700.037.53978349
CACGGCT97950.036.55319631
ATGCCGT97950.036.3396647
AGTCACG102450.035.18707328
GTCACGG103000.035.16885429
GCTACAT100650.034.0437935
CTACATC102400.031.58223536
TGCCGTC118800.030.16777248
CGGCTAC122750.029.51120433
ACGGCTA124100.029.10506632
CATCTCG123550.026.71342539
ACGTCTG141500.026.11869215
Produced by FastQC (version 0.11.5)