FastQCFastQC Report
Tue 16 Apr 2019
H7K37BGXB_n01_1_NLP7_ab_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K37BGXB_n01_1_NLP7_ab_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17371424
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1082460.6231268087175812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC31150.024.1571746
TGGGGGG50350.023.8476311
GTATGCC31800.023.55339447
GCCGTCT36150.021.30032551
CCCGTCC37150.020.9147933
CACCCGT38050.020.69585831
GTCACCC40000.020.47447429
AGTCACC39750.020.339128
TCTCGTA32650.020.1529143
ACCCGTC40000.019.51205432
TATGCCG39050.019.3595248
CCGTCCG41700.018.5489534
GTCCGCA43050.018.2112836
CGTCCGC44400.017.4997535
TCGTATG45200.017.26755345
CCGTCTT44550.017.04831152
TCCGCAT45500.016.53841637
CTCGTAT42750.016.45590644
ATCTCGT40850.016.193342
ATGCCGT47150.016.10807649
Produced by FastQC (version 0.11.5)