FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7248217
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5306317.320848699756091No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC77600.1070608123349508Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG76450.061.67855518
CGTGTGC54700.059.5350917
AGCACAC79300.059.26435517
ACGTGTG56300.058.8722416
AGAGCAC92200.058.75269715
CACACGT58350.058.6612319
GTGACTG52500.058.141591
TGACTGG54450.057.076872
GTGTGCT56650.056.58080718
GGAGTTC60200.056.299717
AGACGTG60050.055.97724514
CAGACGT59800.055.8800113
GACGTGT60550.055.36005415
ACTGGAG58350.055.3068924
CACGTAG35600.055.06911521
GTGCTCT50500.054.90591420
TCAGACG61450.054.83226412
ACACGTC27200.054.70226720
AGTTCAG64550.053.629479
GAGTTCA66500.052.6564038
Produced by FastQC (version 0.11.5)