FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_976.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_976.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13331678
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2058441.5440216902928499No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC178330.13376410681386094Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG26700.039.5841318
AGCACAC38800.029.85520617
CGGGGAC13100.029.6720521
GAGCACA48750.029.28932416
CGGGGTT21850.028.3672811
AGAGCAC52800.028.23544115
CGGGGAG15200.026.7245451
CGGGGTG17300.026.1119611
GGGGTTA33900.024.5793532
CACACGA22850.023.73946419
CACACGT23150.023.12947819
ACACGAG22450.022.91603320
GGGGGAT28650.022.2455251
CACGTAG19900.021.98342521
GCGGGTG20300.021.2180251
GGGGGTA32600.020.8391511
CGGGGAT24700.020.557341
CGGGTGA20750.020.0780752
CACGAGA26900.019.77560421
TGGGGTT52500.018.8765661
Produced by FastQC (version 0.11.5)