FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_916.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_916.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14139076
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2892912.046038934934645No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC244880.17319377871651584Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTA39450.029.9146351
GCACACG21850.026.58808518
GGGGGTC21350.024.7673681
CGGGGTG16300.023.2026141
GGGGGAT30400.022.9234661
CGGGGTT26100.022.8091561
TAGATCG97550.021.8842376
CAGATCG101050.021.8535446
GAGGGTA44800.020.7142221
GGGGTTA34550.019.9614892
AGATCGG345600.019.9186337
GATCGGA347250.019.8441418
GGGGTAA42300.019.0354122
CGCCGTA211650.018.82203353
TCGGAAG358150.018.75162310
GAGCACA48350.018.67450116
GGTCGCC224350.018.63033950
ATCGGAA368850.018.6251349
CCGTATC213900.018.42622455
TCGCCGT216500.018.40038552
Produced by FastQC (version 0.11.5)