FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_768.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_768.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12186500
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3089012.534780289664793No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC179110.14697411069626226Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG27250.037.630818
GAGCACA55300.028.73235916
AGCACAC38950.028.3935817
AGAGCAC56800.028.21958415
CGCCGTA187900.026.91873753
CCGTATC187450.026.1226655
CAGATCG102400.025.8724926
GCCGTAT203550.025.7602954
TCGCCGT196400.025.70025352
TAGATCG109100.025.630936
GGTCGCC205850.025.20060550
GTCGCCG202150.025.15970251
CGTATCA196450.025.0149856
GATCGGA358100.024.8141238
AGATCGG361400.024.5875427
CACACGA21400.023.8770919
GTGGTCG218900.023.82616448
TCGGAAG366700.023.71679710
CTCGGTG198200.023.71609744
ATCGGAA373600.023.625389
Produced by FastQC (version 0.11.5)