FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_731.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_731.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13318233
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2452691.8416031616206145No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC388210.29148761701345816Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG60650.047.7769218
AGAGCAC102600.041.68094615
GAGCACA100150.041.4777416
AGCACAC77700.040.4905817
CACACGA40450.037.98084319
ACACGAG42950.035.85358420
CACACGT38750.034.67992419
CACGAGA46400.033.338621
CACGTAG36600.032.3205521
ACACGTA44050.026.6159520
GATAGAT104850.026.0343288
CAGATCG162100.025.4537056
AAGAGCA196100.025.1804314
GATCGAG28600.025.0846528
AGATAGA114200.023.9334417
ATAGATC114700.023.6155369
TAGATCG172000.022.177796
ACGAGAT71300.021.74489222
CGGGGTT26050.020.972111
CGGGGAC13200.020.959381
Produced by FastQC (version 0.11.5)