FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_1394.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_1394.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11432517
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2570502.248411264116205No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC199970.17491336334772126Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22050.032.2192318
GATCGAG26250.025.4643028
AGATAGA99450.024.9499197
GATAGAT98900.024.840978
ATAGATC100350.024.5169079
CAGATCG159100.023.6024596
GGGGGTA42100.022.0434881
TAGATCG168650.021.7056646
AGAGCAC53850.021.44610215
CGCCGTA182500.021.34845453
GAGCACA53650.021.26540816
TCGCCGT186450.020.87740552
GGTCGCC225350.020.78416850
GCCGTAT217200.020.77424854
GTCGCCG225500.020.49092351
CCGTATC190250.020.38564755
ATCTCGG223150.020.15493642
CTCGGTG240350.019.96522544
TCTCGGT236650.019.87804643
GATCGGA598500.019.7467048
Produced by FastQC (version 0.11.5)