FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_1215b.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_1215b.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8508623
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1054111.2388726119373252Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG874211.0274400452341115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG151300.057.57140418
CACACGA83300.055.91828519
AGAGCAC220950.054.45436515
GAGCACA214050.054.2477816
ACACGAG86400.054.07557320
AGCACAC174950.052.8093517
CACGTAG68200.052.5471521
CACGAGA90700.051.43472721
CACACGT80500.050.16845719
ACACGTA69250.050.1332120
GCGGCGC18500.048.836791
AAGAGCA266850.048.01239414
GATCGAG47100.047.1074078
AGATAGA170500.046.2647677
GATAGAT173050.045.320128
GCAGATC87050.044.8256845
ACGAGAT104900.044.33871522
ACGTAGA84400.042.04642522
CAGATAG75150.041.4459276
ATAGATC199000.039.498229
Produced by FastQC (version 0.11.5)