FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_1214.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_1214.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10514616
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1027490.977201640078915No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC214440.20394468043340813Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG32150.043.9771418
AGCACAC45850.033.35559517
GAGCACA59250.033.19514516
AGAGCAC60500.032.971915
CACACGA24450.028.62712319
CACGTAG22450.027.74870121
CACACGT26600.026.70797519
CAGATCG75550.026.5429296
ACACGAG27300.026.2802920
CCCGGAC13050.024.40380318
GATCGGA255650.023.1623828
AGATCGG258600.023.0064227
CACGAGA31850.022.74573121
GCCCGGA14500.021.96341917
TAGATCG88850.021.9394846
ACACGTA28600.021.90412920
TCGGAAG265350.021.77492310
ATCGGAA273450.021.5138659
TGCCCGG14900.021.37379816
CGCCGTA136850.021.30738353
Produced by FastQC (version 0.11.5)