FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_1204.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_1204.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10094361
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1400331.387239865901368No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC273150.27059662320378675Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18350.028.98932618
GGGGGTA27000.026.3272861
CAGATCG85400.025.4077746
CGGGGTG12550.024.5534631
CCCGGAC18200.023.07493418
GAGCACA40050.022.80702416
TAGATCG90150.022.51626
AGAGCAC41100.022.47970415
ATAGATC74250.022.2944879
TCCGCAC19700.021.67755549
GATAGAT77550.021.6616848
TGCCCGG20300.021.3774716
GCCCGGA19750.021.2639917
GATCGAG20100.021.0679428
CCGCACC20800.021.0360150
GGGGGAT22150.021.025761
AGATAGA81950.020.2851147
GAGCGGC21800.020.23092361
CCGGACA21400.019.95155319
CGGGGTT19150.019.7482571
Produced by FastQC (version 0.11.5)