FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_1203.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_1203.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9980148
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1997152.001122628642381No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC295170.2957571370685084Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG24150.036.66248718
GAGCACA48500.027.85235216
AGAGCAC49900.027.70197515
AGCACAC35900.026.90487517
CAGATCG115800.026.8066336
CCCGGAC14400.026.24700518
GATCGAG24700.025.786928
GGGGGTA24950.025.6839871
TAGATCG124700.025.0056696
GCCCGGA15800.023.47821817
TGCCCGG15400.023.40630516
GATAGAT83500.022.9677878
ACACGAG20350.022.87277420
CACACGA20450.022.7602419
ATAGATC85750.022.7324439
CGGGGTG11200.022.5110821
AGATAGA85800.022.4744667
AAGGGGG77000.021.81580467
CGGGGTT21700.021.784921
CACACGT19750.021.44059419
Produced by FastQC (version 0.11.5)