FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_1091.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_1091.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12582814
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2716752.1590957316860915No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC318460.25309124016297146Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG78050.051.0274218
GAGCACA113850.046.6321116
AGCACAC90850.046.457417
AGAGCAC116200.046.44180315
ACACGAG47250.041.92366820
CACACGA47400.041.56849719
CACACGT48650.041.21981419
CACGTAG42300.040.78976421
CACGAGA53250.037.72566621
ACACGTA46350.036.7725620
GATCGAG31600.028.9058638
AAGAGCA208350.028.72323814
CAGATCG163200.028.2636226
ACGAGAT73850.027.34449622
CGTAGAT65700.026.4217123
GATAGAT102550.026.2094618
AGATAGA108800.025.089867
TAGATCG191750.024.4751836
ATAGATC111200.024.3909939
CGAGATC82400.024.16738723
Produced by FastQC (version 0.11.5)