FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_1028.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_1028.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11139090
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1251251.1232964272665003No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC206080.18500613604881547Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG34750.042.90342718
AGAGCAC64250.033.28123515
GCGGCGC12450.032.3472061
GAGCACA63350.032.262916
AGCACAC49200.032.22332417
CACACGA26600.030.1294419
CAGATCG72150.028.5218836
ACACGAG28650.028.21827320
CACACGT26300.027.14636419
CACGTAG23150.026.45636221
CGCCGTA133900.026.27281253
GATCGGA274800.026.0188968
AGATCGG276650.025.8575537
TCGCCGT141350.025.0366652
CCGTATC139350.024.74154955
GCCGTAT146350.024.63540554
CGGCGCT21750.024.6343141
TAGATCG96100.024.4363526
GGTCGCC155050.024.40478350
TCGGAAG287400.024.37892310
Produced by FastQC (version 0.11.5)