FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_1003.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_1003.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13313693
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1882421.4138977066693668No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC278910.20949108560637536Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG50350.047.68123618
AGCACAC62400.041.05326517
AGAGCAC82150.039.9158215
GAGCACA81900.039.61111516
ACACGAG37050.034.28870420
CACACGT33550.034.005419
CACACGA37800.033.42244719
CACGTAG30600.032.8241221
CACGAGA43350.029.46703121
ACACGTA34600.028.8271320
CGGGGAG16050.026.8382871
CGGGGAC16500.026.1063331
CGGGGTG16500.025.4695931
CAGATCG136550.024.9625236
GGGGGAT27700.024.0214121
TAGATCG138900.023.0788656
GATCGAG26200.022.7074748
CGGGGTT24300.022.4824181
AAGAGCA178100.022.40030714
GGGGGTA36100.022.3123511
Produced by FastQC (version 0.11.5)