FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n02_1002.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n02_1002.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13068464
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2027981.5518120568721772No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC637510.48782320554274783Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG103700.053.79396418
AGCACAC117350.050.1312617
GAGCACA153050.049.25341816
AGAGCAC155500.049.08505215
CACACGA57300.048.3720519
ACACGAG60600.045.7398620
CACACGT64150.043.91604219
CACGTAG54500.043.47448321
CACGAGA68100.040.54825621
ACACGTA58350.040.48602720
GATCGAG40550.035.9892278
AAGAGCA242950.034.42745614
ACGAGAT87300.031.79078122
GATAGAT137850.031.5567048
AGATAGA140200.031.1525737
CGTAGAT78100.030.69600123
ATAGATC143050.030.1894079
ACGTAGA84200.028.2227922
GCACAAG59250.027.28837218
CAGATCG221350.027.2574396
Produced by FastQC (version 0.11.5)