FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7248217
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA450840.6220012452717683TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243760.33630339709752066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT153150.043.5224151
GTGACTG21050.036.2260741
TGACTGG23750.031.949672
TCCGCTC44900.029.47528313
ACGTGTG243000.027.4907532
GCCTGGG15500.027.15899765
GGCCTGG17750.026.26092164
TTCCGCT50750.026.14572512
CTTCCGC50950.026.04309311
GGGTGGC16700.025.81656670
GGGTTGG27900.025.58599570
GGAGTTC21000.025.406187
ACTGGAG30100.025.2094574
AGGCCTG20300.024.13519563
CCTGGGG17650.023.8530366
TAGGCCT23900.023.74183562
TCACAGA166600.022.96157831
CGATCTA76350.022.84838515
CGTGTGC294000.022.769093
CTGGGGG24300.022.71718467
Produced by FastQC (version 0.11.5)