FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_976.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_976.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13331678
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG25750.019.5849341
CGTGTGC34950.018.6381441
CCCCGGG35250.014.2975497
TGCCCCG35350.014.2571045
GTGTGCT47250.013.9998932
CGGCGTG15100.013.90696958
GTGCCCC37850.013.8702294
CCCGGGT37400.013.6627928
CGCGCGG36800.013.41188469
GACGTGT16900.012.6409571
TGTGCTC53700.012.4485083
CCGGGTA45400.012.2574329
AACGCGC40600.012.24284767
ATCGCGG22400.012.1872741
GCGCGGG42150.011.95868570
GTGCTCT61350.011.1814914
GCCCCGG46700.011.0918266
AATCGCG33150.011.08580546
GCGGCGT15200.011.05238257
GGGTGTG32800.010.88390154
Produced by FastQC (version 0.11.5)