FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_916.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_916.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14139076
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTG33300.019.23386458
TATCGCG65250.017.1644846
TGCCGGC26800.016.45490655
CACTAGC286050.016.19965238
ACTAGCT291400.015.96228639
TAGCTTA291150.015.85583841
GCCGGCG18450.015.74495256
GCGGCGT28700.015.73140457
CAGTCAC298450.015.59695234
GCTTATC282100.015.54566243
GCACACG309000.015.52882618
CTAGCTT299500.015.50726840
TCACTAG302050.015.503756537
CCGGCGT24000.015.458034557
CCAGTCA301700.015.44053733
AGTCACT302300.015.40989235
ACACGTC311250.015.40532520
CACACGT311800.015.38937619
GTGTGGG46850.015.38929862
ACGTCTG313450.015.33069722
Produced by FastQC (version 0.11.5)