FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_768.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_768.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12186500
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGCG61700.024.8456246
GCGGGGT50600.023.03321557
ATCGCGT37150.022.98750747
GGGTGGA40200.022.98658266
CGGCGTG25350.022.64262258
CGGGGTG62200.022.50766658
GTGCGTG19900.021.98492264
GTGTGGG39100.020.85643462
ATCGGGG66800.020.64337247
CATCGGG97300.020.6111746
CAGATCA269500.020.57108941
AGTCACC278450.020.4629435
GCCGGCG15850.020.31512356
CAGTCAC281950.020.25857734
ATCGCGG34050.020.24929847
TGCCGGC23150.020.10765855
GATCATC246850.020.03410343
CCAGTCA287850.019.87981833
TGGCGGG53650.019.83191755
TCCAGTC291250.019.7799332
Produced by FastQC (version 0.11.5)