FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_731.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_731.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13318233
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG453300.340360466737592TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG46550.032.4993551
CGTGTGC73250.025.481811
GTGTGCT85500.021.7776782
CCCCGGG25400.018.739797
CCCGGGT26000.018.4419468
CGCGCGG25550.018.08414569
GCGCGGG26000.018.0404170
GTGCTCT107050.017.7857324
TGTGCTC105900.017.7805773
ACGCGCG26400.017.5018968
TGCCCCG28250.017.097015
GTGCCCC30800.015.908784
AACGCGC29050.015.78483767
CCGGGTA36050.015.3395049
GACGTGT24100.015.2574841
CTGCTTG199600.014.48368458
TGCTTGA204750.014.4441659
GCTTGAA214100.014.4345660
GCCCCGG34300.014.1833956
ATGCCGG34300.013.57289470
Produced by FastQC (version 0.11.5)