FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_1394.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_1394.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11432517
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG542220.47427876118618495TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA263200.021.90402666
TGCTTGA261300.020.87104865
ACGTGTG24950.020.7728211
CTGCTTG258550.020.29204664
GCGTGAA54650.020.17607166
GCCGTCT315650.019.33748256
CTTGAAA309300.019.18253367
TGCCGTC361500.018.99546455
ATGCCGT374450.018.66566554
CCGTCTT333550.018.57255257
TGCGTGA43100.018.51713665
TATGCCG388450.018.4254253
CATCTCG399550.018.06253246
GTATGCC405750.017.99347352
CTCGTAT410800.017.55928449
CGTATGC415450.017.4806951
ATCTCGT412600.017.4572347
CTGCGTG42350.017.43804464
TGAAAAA442900.017.27661969
CGTCTTC343200.017.2752958
Produced by FastQC (version 0.11.5)