FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_1215b.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_1215b.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8508623
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG191670.22526559232909954TruSeq Adapter, Index 3 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATG100460.11806845831575802TruSeq Adapter, Index 3 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG112850.046.0803531
GACGTGT36600.036.5488051
CGTGTGC218800.027.7358911
GTGTGCT219550.027.5466582
CGTGAAA83500.024.98545667
GCGTGAA79200.024.97185366
AAAAGGG82400.024.7242670
TGTGCTC246550.024.5583783
GTGGAAA115150.024.2586468
GTGCTCT252350.024.174234
TGCGTGA61200.023.10773865
GTGCGTG54500.023.05535964
CAGGGGG23850.023.04014464
GCCGGCT30850.022.68994556
TGCCGGC66550.022.61408255
GTGAAAA154900.022.23674468
GCAGGGG25700.022.06177763
GGGTGGA83800.022.05552566
CCGGCGT50600.021.9955757
GGTGGAA87450.021.97556367
Produced by FastQC (version 0.11.5)