FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_1214.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_1214.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10514616
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG259880.24716071419060856TruSeq Adapter, Index 1 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG30500.025.7200551
CCCCGGG12400.021.1689287
CGCGCGG12900.020.89353869
GCCGTCT141750.020.88846856
TGCCGTC151550.020.6693355
CGTGTGC40350.020.3093511
ACGCGCG12950.020.0020768
CCCGGGT13850.019.9634978
TATGCCG157750.019.9235353
ATGCCGT160400.019.48526854
GCGCGGG14050.018.93425470
CACGATC159200.018.79682243
CCGTCTT159600.018.72769557
GATCTCG156550.018.71258446
GTATGCC172600.018.39186752
CTGCTTG148200.018.25562964
GCTTGAA158150.018.2377466
CGATCTC162350.018.0656345
ATCTCGT168600.017.54116647
ACGATCT169500.017.53070444
Produced by FastQC (version 0.11.5)