FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_1204.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_1204.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10094361
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG262970.26051178474793996TruSeq Adapter, Index 13 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT128800.016.73884856
TGCCGTC153400.016.24483555
GCGTGAA40000.016.01405366
TATGCCG174650.015.73119853
ATGCCGT164050.015.68092954
CCGTCTT143800.015.40655457
GTATGCC185150.015.27384452
CTGCTTG114450.015.22940264
GCTTGAA134600.015.16120366
CTCGTAT187000.014.91686149
TGCTTGA128450.014.52456965
CGTATGC192450.014.51261551
AATCTCG209950.014.2031446
ATCTCGT203600.014.1991747
CGTGTGC25050.014.1182511
TCTCGTA199150.013.98921948
TCGTATG200300.013.8040650
ACGTGTG18050.013.7736291
CACAGTC264450.013.73771538
GTCACAG264300.013.73226836
Produced by FastQC (version 0.11.5)