FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_1203.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_1203.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9980148
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG134810.13507815715758925TruSeq Adapter, Index 12 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA50550.021.88225266
ACGTGTG21950.020.4209371
TGCGTGA43800.019.42040365
CGTGAAA61600.019.09345867
GCCGTCT89850.018.2689456
TATGCCG153250.017.85931853
TGCCGTC121100.017.51406955
CTGCGTG39300.017.27715164
ATGCCGT133450.017.23079754
GTATGCC165450.017.19817552
GTGCGTG24950.017.11402764
CTCGTAT128900.016.61717249
CGTATGC171000.016.53765151
TGCCGGC37200.016.37061155
CCGTCTT109850.016.15349657
ATCTCGT146850.015.92066147
TGTAATC205100.015.90409143
GCCGGCG22950.015.86022156
TCTCGTA136900.015.79951148
AATCTCG154450.015.7264346
Produced by FastQC (version 0.11.5)