FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_1091.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_1091.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12582814
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG389590.30962072553881825TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG54100.037.8665281
CGTGTGC91000.025.936741
GTGTGCT100300.023.7638572
TGTGCTC117300.020.3491733
GACGTGT27550.019.574741
GTGCTCT123200.019.4598854
CGCGCGG24250.017.17679869
TGCCCCG24750.017.110815
CCCCGGG25150.016.4211837
CCCGGGT25850.016.2472978
GCGCGGG25650.016.23927370
CCGGGTA32950.015.7205129
ACGCGCG26800.015.67304868
GTGCCCC27750.015.5132414
CTGCTTG157500.014.86669964
TGCTTGA167050.014.83521365
GCGTGAA45200.014.71370966
GCTTGAA179400.014.69195466
AACGCGC28100.014.57426267
GCCGTCT198300.014.40216756
Produced by FastQC (version 0.11.5)