FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_1028.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_1028.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11139090
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG35100.027.1376421
TATCGCG59600.023.19591546
ATCGCGT39450.022.6231947
CGGCGTG25100.021.1948658
TGCCGGC30400.020.83846355
TATGCCG46900.020.07428753
CGTGTGC52850.019.9448971
TCGCGTA28350.019.62929548
GCCGGCG17850.019.2153656
CCGATGT223650.019.07635940
CGATGTA228600.018.7398441
GTATCGC77950.018.67832445
CCGGCGT23050.018.52463257
ACCGATG232200.018.4945239
GCGTGAA31050.018.48774366
GCACACG255400.018.48637418
ACACGTC258550.018.31529620
CACACGT259000.018.31050119
TAGATCG63350.018.2317376
CACCGAT237050.018.16041838
Produced by FastQC (version 0.11.5)