FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_1003.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_1003.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13313693
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG353430.26546353442279313TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG38200.032.0889431
CGTGTGC55650.027.1875021
GTGTGCT67450.022.416712
TGTGCTC84050.018.197113
GTGCTCT87900.017.559354
GCGCGGG37550.017.52522970
CGCGCGG39650.016.5970369
TGCCCCG41500.016.3610925
CCCGGGT41450.015.9586428
ACGCGCG41800.015.74335668
CCCCGGG42300.015.4724797
GCTTGAA209550.014.86682166
CCGGGTA49200.014.6541559
GTGCCCC45950.014.6242764
AACGCGC44700.014.56535967
TGCTTGA209400.014.55986165
CTGCTTG209300.014.28062864
ATGCCGG18100.013.92418270
GACGTGT21850.013.3038511
GCCCCGG52800.012.7932846
Produced by FastQC (version 0.11.5)