FastQCFastQC Report
Wed 19 Dec 2018
H7K2KAFXY_n01_1002.rh2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2KAFXY_n01_1002.rh2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13068464
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG133400.10207779582971648TruSeq Adapter, Index 5 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG74950.038.0795171
GACGTGT33950.024.5494981
CGTGTGC135600.023.7334691
GTGTGCT137050.023.444072
TGTGCTC158650.020.2076823
GTGCTCT166600.019.2223834
GCGCGGG23800.016.913570
CGCGCGG23200.016.89828769
GCGTGAA62500.016.6316760
CTGCGTG48950.016.15902558
ACGCGCG24150.015.79872268
TGCGTGA53250.015.57715659
GGGGGTG74900.014.90807368
GGGTGGA48350.014.84123470
CGTGAAA75700.014.79495161
TATGCCG145050.014.67051347
CCCCGGG26500.014.6601437
GGGTGGC43800.014.62478970
GTGCGTG39850.014.49333368
ATGCCGT110200.014.228394548
Produced by FastQC (version 0.11.5)