FastQCFastQC Report
Sat 13 May 2023
H7K2JDRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2JDRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981337936
Sequences flagged as poor quality0
Sequence length24
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGGTTGGCGTGTAGATCTCGGT23277660211.748455312471238No Hit
TTCAGTCTAAGTGTAGATCTCGGT1832281119.247696098218753No Hit
TGGTAACACTGTGTAGATCTCGGT1314370106.6337502357296No Hit
ATCAGTCTAAGTGTAGATCTCGGT1290170466.511612363333865No Hit
AGGTAACACTGTGTAGATCTCGGT902183784.55340688535628No Hit
GATGAAGATAGTGTAGATCTCGGT887961054.4816234215585125No Hit
TGATCTCGGTGGTCGCCGTATCAT212970471.0748821093586531Illumina Single End PCR Primer 1 (95% over 24bp)
AGATCTCGGTGGTCGCCGTATCAT145995940.7368553205756617Illumina Single End PCR Primer 1 (100% over 24bp)
GAGGGTTGGCGTGTAGATCTCGGT93720930.47301839982535926No Hit
GGGGGGGGGGGGGGGGGGGGGGGG66702670.33665468564470064No Hit
TGGGAACACTGTGTAGATCTCGGT56324650.2842758369312321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)