FastQCFastQC Report
Sat 13 May 2023
H7K2JDRX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2JDRX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981337936
Sequences flagged as poor quality0
Sequence length10
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTACGAAA31985072816.143168824886416No Hit
GTGGATCAAA25458122212.848955111310199No Hit
GTAACATGCG23456631711.838783921613663No Hit
CTCTAGCGAG922517564.656033396616901No Hit
CCTACCATAT871164464.396849442850419No Hit
AGCCCTTTAT840446184.241811377703314No Hit
CAAGTCCAAT831331204.1958072113549845No Hit
TGTAGCGGAT786483283.9694555164465393No Hit
AAACGGCGAT774666253.9098138481309532No Hit
TTGTAAGAAT772518993.8989764237775137No Hit
CTGCAGCCAT755347213.812308825645985No Hit
TCTTAGGCAT754111743.806073291679002No Hit
GGCGTTTCAT736845273.7189277841596833No Hit
AAAGCATAAT659172433.3269056127333947No Hit
GTGAGAAGAT641645203.2384440248258586No Hit
GGGGGGGGGG551181772.78186653566401No Hit
GCCTTTATAT343231171.7323201850812389No Hit
TCGCAATTAT211408131.0669968315793656No Hit
AGAACGCCAT188883840.9533146091238016No Hit
CATGGCAGAT180140290.9091850851232073No Hit
GTCTTTGAAT168610710.8509942041507451No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)