FastQCFastQC Report
Sat 4 Mar 2023
H7K2HBGXN_n01_JK161b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2HBGXN_n01_JK161b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17583852
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCC3321991.8892276845824225No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1842681.047938756536395TruSeq Adapter, Index 5 (100% over 50bp)
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT721960.41058125375486554No Hit
GCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGC625030.3554568134445172No Hit
AGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAG485470.27608853850680726No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG438020.2491035525094274No Hit
TAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTA433230.24637946224752122No Hit
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT398850.22682743235100025No Hit
TTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTT368190.20939097986038555No Hit
AGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAG298620.1698262701483156No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC291950.16603301711138152No Hit
AAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAA286560.16296770468723235No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC260830.14833496096304724TruSeq Adapter, Index 5 (98% over 50bp)
TAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTA202000.1148781279551261No Hit

[OK]Adapter Content

Adapter graph