FastQCFastQC Report
Sat 4 Mar 2023
H7K2HBGXN_n01_JK159b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7K2HBGXN_n01_JK159b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13209219
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCC1525011.1545042897691378No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC895350.6778220574585069TruSeq Adapter, Index 3 (100% over 50bp)
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT352320.2667228092743409No Hit
GCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGC311820.23606240459787972No Hit
AGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAG232970.17636924635741144No Hit
TAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTA231120.17496870935367184No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG225810.17094878962942472No Hit
TTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTT206690.15647405043401885No Hit
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT164690.12467807521398502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)