FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51802952
Sequences flagged as poor quality0
Sequence length110
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14063662.7148375636971425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1252950.084.1966252
AAGCAGT1513950.069.998421
GTATCAA1649700.063.5083169
GCAGTGG1844450.058.200153
GGTATCA1870750.056.405048
CAGTGGT1924550.055.140264
AGTGGTA1966900.054.1951945
GTGGTAT2031400.052.423946
TGGTATC2255100.046.8983657
ATCAACG1662450.035.91338710-11
AGAGTAC1671650.035.48992518-19
TACATGG1663300.034.6354522-23
AGTACAT1743950.033.67857420-21
CATGGGG519750.032.34682524-25
CATGGGA1118200.031.81362524-25
CAGAGTA1689300.031.39791118-19
CAACGCA1915850.031.3932912-13
CGCAGAG1716450.031.27965716-17
GCAGAGT1968900.030.50401116-17
ACATGGG1632000.030.18976624-25
Produced by FastQC (version 0.11.5)