FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_H1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_H1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18991133
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC570140.3002137892457496No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA393480.20719142981095443No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT346530.18246936609837863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1958300.097.2631151
AGCAGTG2190100.086.81422
GCAGTGG2553100.074.335443
CAGTGGT2723700.069.6349644
AGTGGTA2773750.068.4127665
TGGTATC2858550.066.283757
GTGGTAT2881500.065.8091056
GGTATCA2890250.065.6139458
GTATCAA2954300.064.293679
CAACGCA2956150.036.37929512-13
ATCAACG2972350.036.16980710-11
GCAGAGT2985700.035.7920216-17
AGAGTAC2985050.035.71056718-19
ACGCAGA3029900.035.47150414-15
TACATGG3010750.034.74658622-23
AGTACAT3127600.033.9610620-21
CATGGGG881000.033.61780524-25
TCAACGC2935650.032.40944712-13
CATGGGA1989000.031.99551224-25
AACGCAG3006700.031.64370714-15
Produced by FastQC (version 0.11.5)