FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_H1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_H1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24356502
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC728100.29893455143928305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA497540.20427399632344578No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT439850.1805883291451293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG334130.13718308154430384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2505350.097.238451
AGCAGTG2813650.086.415992
GCAGTGG3286550.073.917143
CAGTGGT3494700.069.439134
AGTGGTA3555150.068.265265
TGGTATC3657600.066.244837
GTGGTAT3688250.065.7525256
GGTATCA3694850.065.650518
GTATCAA3780200.064.28499
CAACGCA3779550.036.47165712-13
ATCAACG3798950.036.29020310-11
GCAGAGT3814700.035.9026816-17
AGAGTAC3812950.035.8753118-19
ACGCAGA3870800.035.60319514-15
TACATGG3842400.034.8839522-23
AGTACAT4000700.034.0577720-21
CATGGGG1129300.033.63669624-25
TCAACGC3751700.032.406812-13
CATGGGA2530400.032.18345624-25
AACGCAG3842450.031.6591414-15
Produced by FastQC (version 0.11.5)